BIOCHEMISTRY: TCA CYCLE, GLUCONEOGENESIS, ENZYME KINETICS

DIRECTIONS : PLEASE CAREFULLY READ ALL QUESTIONS AND ANSWER ALL QUESTIONS.OUTLINES ARE ATTACHED TO THIS POST AS A REFERENCEDUE TODAY: @ 6:00PM EASTERN STANDARD TIME1.) The equilibrium constant of an uncatalyzed reaction Keq is 0.5. If an enzyme having a Km of 0.01M and Vmax of 0.1 umoles/min is used to catalyze this same reaction, the Keq of the catalyzed reaction would be:A.  5B. 0.005C. 0.5D. 0.052.) Addition of a competitive inhibitor to an enzymatically catalyzed reaction willA. increase the affinity the enzyme has for its substrateB.  reduce the Vmax of the reactionC. increase the substrate concentration required to attain ½ Vmax3.) The equation below describes the behavior of which of the following reactions obeying Michaelis-Menton kinetics?Vo = Vmax [S] / (Km + a’ [S])A. An uninhibited reactionB. A non competitively inhibited reactionC. None of the above answers are correctD. competitively inhibited reaction4.) Which of the following enzymes covalently modifies the FBP-2/PFK-2 enzyme complex?A. PFK-2B. protein kinase AC. pyruvate kinaseD. PFK-15.) For each time (cycle) of the TCA cycle , how many molecules of ATP equivalents are produced ?A. 24B. 18C. 6D. 126.) The first reaction of the tricarboxylic acid cycle condenses the acetyl group,  acetyl CoA with which of the following TCA cycle intermediates ?A. pyruvateB. oxaloacetateC. citrateD. malate7.) In the TCA cycle  TP is produced from CDP and Pi by which of the following mechanisms?A. substrate level phosphorylationB. cAMP- dependent kinase phosphorylationC. reverse phosphorylation8.) Succinate thiokinase (AKA succinyl – CoA synthetase) cleaves a high energy bond CoA this high energy bond is of which of the following types ?A. EsterB carboxylic acidC.aldehydeD. thioester9.) The primary substrate in gluconeogenesis is which of the following  ?A. glicerolB. fatty acidsC. amino acidsD. glucose10.)Which of the following results in a futile cycle ?A.  glycolysis is inactive and glycogenesis is activeB.  both glycolysis and gluconeogenesis are inactiveC.  both glycolysis and gluconeogenesis are activeD.  glycolysis is active and gluconeogenesis is inactive11.) True/ False:  Oxaloacetate is permeable to inner mitochondrial membraneA.TrueB.False12.)The Cori Cycle is best described as which of the following?A. glucose  converted to pyruvate and back to glucoseB. glucose converted to glycogen and back to glucoseC. glucose converted to ATP and back to glucoseD. glucose converted to lactate and back to glucose13.) True /False the reaction : Acetyl – CoA+ oxaloacetate -> citrate is an anaplerotic reactionA.TrueB.False14.) When initial reaction velocity is plotted as a function of substrate concentration the kinetics data of non allosterically regulated enzymes generate a curve withA. linear morphologyB.  Hyperbolic morphologyC.  zero order morphologyD. sigmoidal morphology15.) Addition of a competitive inhibitor to an enzymatically catalyzed reaction willA. increase the affinity the enzyme has for its substrateB.  reduce the Vmax of the reactionC. increase the substrate concentration required to attain ½ Vmax